Journal: ACS Omega

Article Title: OrganoLabeler: A Quick and Accurate Annotation Tool for Organoid Images

doi: 10.1021/acsomega.4c06450

Figure Lengend Snippet: Immunochemical characterization of embryoid bodies and 30 day old brain organoids generated under three-different conditions. (A) Embryoid bodies stained to demonstrate the presence of three distinct germ layers; with α-SMA (mesodermal marker), Nestin (ectodermal marker), Sox17 (endodermal marker), and DAPI (cell nucleus). (B) Brain organoid generated under static condition stained with Sox2 and Tuj1. (C) Brain organoid generated under static condition stained with N-cadherin. (D) Brain organoid generated using an orbital shaker stained with Sox2 and Tuj1. (E) Brain organoid generated using an orbital shaker stained with N-cadherin. (F) Brain organoid generated using a microfluidic chip stained with Sox2 and Tuj1. (G) Brain organoid generated using a microfluidic chip stained with N-cadherin. (Right panels: magnification of dashed zones).

Article Snippet: Subsequently, primary antibodies (α-SMA (Cell Signaling Technology, Cat no.48938S), Nestin (Proteintech, Cat no. 19483-1-AP), Sox17 (Abcam, Cat no. Ab84990), Sox2 (Bioss, Cat no. bs-0523R), Tuj1 (R&D Systems, Cat no. MAB1195), and N-cadherin (Cell Signaling Technology, Cat no. 13116T) were added according to related samples and incubated for overnight at 4 °C.

Techniques: Generated, Staining, Marker

Journal: Acta physiologica (Oxford, England)

Article Title: Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing

doi: 10.1111/apha.12919

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Tuj 1 (Neurone-specific class III beta-tubulin) 84 , Neurones , Chicken, affinity purified, Neuromics CH23005 , AB_2210684 , 1 : 1000.

Techniques: Purification, Concentration Assay, Marker, Affinity Purification

Journal: PLoS ONE

Article Title: Glucagon-like peptide 1 receptor (GLP-1R) expression by nerve fibres in inflammatory bowel disease and functional effects in cultured neurons

doi: 10.1371/journal.pone.0198024

Figure Lengend Snippet: Double staining of GLP-1R (Abcam GLP-1R antibody at a dilution of 1:1000, black) with the neuronal marker β Tubulin (at a dilution of 1:1000, red) in, A, sub-mucous plexus of colon from a patient with IBD and, B, human DRG; magnification x20.

Article Snippet: For tissue immunohistochemistry, GLP-1R antibody ab39072 (Abcam, Cambridge, UK) was used at a final titre of 1:1000, CGRP antibody (Merck Millipore AB59200, Watford, UK) was used at a final titre of 1:4000 and rabbit polyclonal anti-human PGP9.5 antibody (RA95101, UltraClone Ltd, UK), was used at 1:40,000, and neuron-specific mouse anti-β-II tubulin/Tuj1 (R&D Systems, Inc, Oxford, UK) at 1:100.

Techniques: Double Staining, Marker

Journal: PLoS ONE

Article Title: Glucagon-like peptide 1 receptor (GLP-1R) expression by nerve fibres in inflammatory bowel disease and functional effects in cultured neurons

doi: 10.1371/journal.pone.0198024

Figure Lengend Snippet: Cultured adult rat DRG neurons (A, B, C) showing β tubulin immunofluorescence (green in A), GLP-1R expression (red in B), and the merged image (C). Bar = 200 μm. High power image of cultured human DRG neurons positive for β tubulin (green in D), intense GLP-1R expression (red) in a small neuron (E), and merged image (F). Bar = 20 μm.

Article Snippet: For tissue immunohistochemistry, GLP-1R antibody ab39072 (Abcam, Cambridge, UK) was used at a final titre of 1:1000, CGRP antibody (Merck Millipore AB59200, Watford, UK) was used at a final titre of 1:4000 and rabbit polyclonal anti-human PGP9.5 antibody (RA95101, UltraClone Ltd, UK), was used at 1:40,000, and neuron-specific mouse anti-β-II tubulin/Tuj1 (R&D Systems, Inc, Oxford, UK) at 1:100.

Techniques: Cell Culture, Immunofluorescence, Expressing

Journal: PLoS ONE

Article Title: Glucagon-like peptide 1 receptor (GLP-1R) expression by nerve fibres in inflammatory bowel disease and functional effects in cultured neurons

doi: 10.1371/journal.pone.0198024

Figure Lengend Snippet: Representative images showing β tubulin immunofluorescence in rat DRG neurons cultured with vehicle (A, control), with Oxm (B), exendin-4 (C), or GLP-1 (D). Bar = 100 microns. Graph showing significantly increased neurite lengths after 48 h treatment with oxyntomodulin (*p = 0.04), exendin-4 (*p = 0.01) and GLP-1 (*p = 0.04) using Student’s unpaired t -test (E). Graph showing lack of significant effect of acute treatment with incretin hormones on capsaicin sensitivity in adult rat DRG neurons (F).

Article Snippet: For tissue immunohistochemistry, GLP-1R antibody ab39072 (Abcam, Cambridge, UK) was used at a final titre of 1:1000, CGRP antibody (Merck Millipore AB59200, Watford, UK) was used at a final titre of 1:4000 and rabbit polyclonal anti-human PGP9.5 antibody (RA95101, UltraClone Ltd, UK), was used at 1:40,000, and neuron-specific mouse anti-β-II tubulin/Tuj1 (R&D Systems, Inc, Oxford, UK) at 1:100.

Techniques: Immunofluorescence, Cell Culture